Biography
Alireza Badiei is a trained veterinarian and he worked as a veterinarian before he started his Postgraduate studies in Immunobiology from the School of Medicine, Putra University of Malaysia in 2008. He was appointed as a Lecturer at the University of Malaysia Kelantan, Faculty of Veterinary Medicine. Currently, he is finalizing his PhD in the Department of Pathology, University of Otago, Christ Church (age 39 years). He has published more than 10 papers from his Master’s and PhD projects.
Abstract
Hydrogen sulfide (H2S) plays an important role in inflammation. It is produced by the activity of the enzyme cystathionine γ-lyase (CSE). Research on the role of CSE is confounded by the lack of specificity of pharmacological agents that inhibit CSE. The use of small interfering RNA (siRNA) to inhibit the production of the CSE is more a specific approach. Using a mouse model of caerulein-induced acute pancreatitis, we have observed that higher levels of pro-inflammatory cytokines including TNF-α, IL-6, MCP-1 and MIP-2, as well as increased activity of pancreatic and lung MPO and also plasma amylase levels. We used siRNA to silence gene for expression CSE in monocytes in an in vivo model of mouse acute pancreatitis. Our results show that siRNA treatment attenuates inflammation in the pancreas and lungs of mice following acute pancreatitis and reduces levels of pro-inflammatory mediators including IL-6, TNF-α, MCP-1, andMIP-2. Pancreatic and lung MPO activities as well as plasma amylase levels were reduced following in vivo silencing of CSE with siRNA. These findings show a crucial pro-inflammatory role for H2S synthesized by CSE in acute pancreatitis.
Biography
Abstract
Despite permanent improvement of congenital heart disease surgical technique and perioperative care still significant number of procedures remain complicated or unsuccessful. The research continues into the impact of patient’s individual susceptibility to complications as a consequence of genetically determined possibility to react to wide spectrum of stressful stimuli occurring during surgery: cardiopulmonary bypass (CPB), infection, hypothermia and others. The attention has been paid to the complement system which is strongly involved in response to special conditions generated during cardiac surgery procedure. The problem was intensely investigated in adult population but has been up till now not elucidated in pediatric patients.We investigated the association between MBL serum concentrations, corresponding MBL2 genotypes and the postoperative course of children in whom cardiac surgery with the use of CPB was performed, in a prospective study. Blood/serum samples were collected from 195 patients. The obtained results suggest protective, beneficial effect of low MBL serum levels. The incidence of SIRS was lower in children with preoperative MBL concentrations<1000 ng/ml and point mutations within exon 1 of the MBL2 gene (A/O and O/O genotypes). MBL deficient patients (<100 ng/ml) with very low MBL levels had generally shorter ICU length of stay and lower inotropic score. In contrast, high MBL-producing genotypes (YA/YA) were more common among children who developed SIRS. This work was supported by the Polish National Science Centre (grant UMO-2011/03/B/NZ6/00052).